Genetics of Wound Healing
We have established that adult MRL mice show unique regenerative responses. These include 1) the closure of through-and-through ear holes, 2) the re-growth of cartilage and hair follicles, and 3) the healing of heart tissue after a cryoinjury to the right ventricle without scarring, with the near full replacement of cardiomyocytes, and with return to normal heart function. We have also demonstrated that the ear hole closure phenotype is controlled by multiple genetic loci, behaves as a true quantitative trait, and is profoundly influenced by sex.
We will identify the genes that regulate healing in these mouse strains, using a combination of linkage mapping and gene expression profiling. The inbred MRL mouse strain derives from the LG mouse strain, which to date is the only other mouse strain shown to have this regenerative capacity. A panel of recombinant inbred (RI) lines and an Advanced Intercross Line (AIL) between LG/J (large) and SM/J (small) mice has been constructed. We are examining their ability to regenerate, to explore the full range of their healing phenotypes, and to map the regeneration genes in this MRL progenitor.
Dr. Ellen Heber-Katz, Wistar Institute.
Dr. Libby Blankenhorn, Drexel University
Supported by NIGMS grant GM073226